Coding

Part:BBa_K2984043:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsaD-MHETase-3xHA-RbcS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
    Illegal NotI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2854
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1609
    Illegal PstI site found at 1933
    Illegal PstI site found at 2276
    Illegal PstI site found at 3086
    Illegal NgoMIV site found at 1542
    Illegal NgoMIV site found at 2003
    Illegal NgoMIV site found at 2033
    Illegal NgoMIV site found at 2348
    Illegal NgoMIV site found at 2366
    Illegal NgoMIV site found at 2402
    Illegal NgoMIV site found at 3045
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed on a level 1 c backbone (BBa_K2984002). Together with L1a (BBa_K2984003) and L1b (BBa_K2984005), a level 2 construct can be built for multiple gene insertion.


Source

The construct was built out of 4 different level 0 plasmids using the MoClo standard (listed in the main description). The TU Kaiserslautern team 2019 kindly provided the MHETase for this construct (BBa_K3002037).

References